DISTRIBUTION OF AMINO-ACIDS IN BRAN, EMBRYO AND MILLED ENDOSPERM AND SHIFTS IN STORAGE PROTEIN SUBUNITS OF IN-VITRO-SELECTED AND LYSINE-ENHANCED MUTANT AND WILD-TYPE RICE

被引：7

作者：

SHARPE, FT ^{[1
]}

SCHAEFFER, GW ^{[1
]}

机构：

[1] USDA ARS,BELTSVILLE AGR RES CTR,INST PLANT SCI,BELTSVILLE,MD 20705

来源：

PLANT SCIENCE | 1993年 / 90卷 / 02期

关键词：

RICE; MUTANT; ENDOSPERM; PROTEIN; LYSINE;

D O I：

10.1016/0168-9452(93)90234-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Experiments were designed to test whether an enhanced lysine phenotype from in vitro selections was expressed in milled rice, Oryza sativa L. Analysis of the endosperm tissue after removal of the aleurone and outer layers proved the grains maintained enhanced lysine after milling. Mutant endosperm had 15% greater lysine than the control and the enhanced lysine was correlated with reduced glutamic acid in two different mutants. Glutelins separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed increases in the protein quantity of the 33-kDa alpha and 21-kDa beta subunits.

引用

页码：145 / 154

页数：10