TUMOR-NECROSIS-FACTOR PRODUCTION BY HUMAN GRANULOCYTES

被引：13
|
作者：
MANDI, Y
ENDRESZ, V
KRENACS, L
REGELY, K
DEGRE, M
BELADI, I
机构：
[1] ALBERT SZENT GYORGYI MED UNIV,INST PATHOL,H-6720 SZEGED,HUNGARY
[2] TRIGON BIOTECHNOL,BUDAPEST,HUNGARY
[3] UNIV OSLO,NATL HOSP,OSLO 3,NORWAY
来源：
INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY | 1991年 / 96卷 / 02期
关键词：
D O I：
10.1159/000235479
中图分类号：
R392 [医学免疫学];
学科分类号：
100102 ;
摘要：
Human polymorphonuclear leukocytes kill WEHI 164 cells in an 18-hour Cr-51 release assay. Antibody to human tumor necrosis factor (TNF) blocks the lysis of targets mediated by human granulocytes. Resting granulocytes produce an undetectable amount of TNF, if any. Granulocytes stimulated with Staphylococcus aureus release 250-500 U/ml TNF-alpha. The specificity of the released TNF in the WEHI 164 cytotoxicity assay was confirmed by using neutralizing anti-TNF-alpha monoclonal antibodies. The thymidine uptake of endothelial cells was inhibited by granulocyte-derived TNF. The identity of TNF-alpha was further confirmed by molecular weight determination, by gel filtration on Sephacryl S-200, with a result of approximately 44,000. Besides their antimicrobial capacity, therefore, granulocytes may contribute to tumor rejection, inflammation and septic infections by releasing TNF.
引用
收藏
页码：102 / 106
页数：5
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