THE RNA CLEAVAGE BY HYBRIDASE .2. AUTOMATIC SYNTHESIS OF OLIGO(RIBODEOXYRIBO)NUCLEOTIDE PROBES

被引：0

作者：

ROMANOVA, EA

ORETSKAYA, TS

SUKHOMLINOV, VV

KRYNETSKAYA, NF

METELEV, VG

SHABAROVA, ZA

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来源：

BIOORGANICHESKAYA KHIMIYA | 1990年 / 16卷 / 10期

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摘要：

Several oligo(ribodeoxyribo)nucleotides to be used as probes in the RNase H-induced hydrolyses of 5S ribosomal RNA E. coli were synthesized on the Victoria-4M automatic gene synthesizer by the phosphoramidite approach, which allows for introducing ribonucleotides into any position of the oligomer. 2'-Hydroxy function was protected by tert-butyldimethylsilyl group whose hydrophobicity simplified isolation of the oligonucleotides by reverse-phased HPLC.

引用

页码：1348 / 1354

页数：7

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