ENANTIOMERIC RESOLUTION BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY WITH CHIRAL SURFACTANTS

Enantiomeric resolution of phenylthiohydantoin (PTH)-amino acids by micellar electrokinetic chromatography using chiral surfactants was studied. As a chiral surfactant, digitonin, which is a non-ionic compound, was used with anionic sodium dodecyl sulphate (SDS) to form mixed micelles. Under acidic conditions (pH 3.0), PTH derivatives of six amino acids (tryptophane, norleucine, norvaline, valine, α-aminobutyric acid and alanine) were separated from each other and optically resolved with a 25 mM digitonin-50 mM SDS solution, although a long separation time was required. The use of an anionic chiral surfactant, sodium N-dodecanoyl-l-valinate, was also examined under neutral conditions. In this instance, the same enantiomers as above except PTH-dl-Ala were resolved in a shorter time. © 1990.