Reduced PCR cycling time amplification using AmpFlSTR (R) Identifiler (R) kit

被引：4
|
作者：
Iyavoo, S. [1 ]
Wolejko, A. [1 ]
Furmanczyk, D. [1 ]
Graham, R. [1 ]
Myers, R. [1 ]
Haizel, T. [1 ]
机构：
[1] Anglia DNA Serv Ltd, Norwich, Norfolk, England
来源：
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES | 2015年 / 5卷
关键词：
DNA; PCR; STR; Reduced time; Identifiler;
D O I：
10.1016/j.fsigss.2015.09.113
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
To reduce the DNA profiling time, DNA samples were amplified using AmpFlSTR (R) Identifiler (R) kit at reduced PCR cycling time amplification method without addition of any enhancement buffers or enzymes. The results showed no significant difference in the quality of the profiles produced compared to those produced in the manufacturer recommended condition. This study contributed in saving almost 25% of the time needed for PCR amplification without compromising the quality of the profiles. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：E286 / E288
页数：3
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