REACTIVITY OF MONOCLONAL-ANTIBODIES TO SPECIES-SPECIFIC ANTIGENS OF ENTAMOEBA-HISTOLYTICA

被引:14
|
作者
TACHIBANA, H [1 ]
KOBAYASHI, S [1 ]
NAGAKURA, K [1 ]
KANEDA, Y [1 ]
TAKEUCHI, T [1 ]
机构
[1] KEIO UNIV,SCH MED,DEPT PARASITOL,TOKYO 160,JAPAN
来源
JOURNAL OF PROTOZOOLOGY | 1991年 / 38卷 / 04期
关键词
INDIRECT FLUORESCENCE ANTIBODY TEST; WESTERN BLOTTING;
D O I
10.1111/j.1550-7408.1991.tb01368.x
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Twenty monoclonal antibodies were produced against trophozoites of Entamoeba histolytica strains HK-9 and HM-1: IMSS. When reactivity to various enteric protozoa was examined by an indirect fluorescence antibody test, 15 of the monoclonal antibodies were strongly reactive with E. histolytica trophozoites. Species-specific antigens recognized by these monoclonal antibodies were located on the plasma membrane, nucleus, cytoplasm, and cytoskeletal structures of the trophozoites. Two of the remaining five monoclonals reacted strongly with trophozoites of the E. histolytica-like Laredo strain. The determinant antigen was located in the cytoplasm. The three remaining monoclonal antibodies were found to recognize cross-reactive antigens between E. histolytica and E. histolytica-like Laredo, E. hartmanni, E. coli, Dientamoeba fragilis, Giardia lamblia, and Trichomonas hominis. These three antibodies were also reactive with T. vaginalis and mammalian cells such as HeLa cells. Thus, the combined use of monoclonal antibodies seems capable of distinguishing E. histolytica and/or E. histolytica-like Laredo from other enteric protozoa.
引用
收藏
页码:329 / 334
页数:6
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