Immobilization of myo-Inositol-1-phosphate Synthase Containing Active, Self-Regenerating Coenzyme (NAD(+)) on the Same Matrix

被引：2
|
作者：
Pittner, F. [1 ,2 ]
机构：
[1] Univ Vienna, Inst Allgemeine Biochem, A-1090 Vienna, Austria
[2] Ludwig Boltzmann Forsch Stelle Biochem, Vienna, Austria
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1981年 / 6卷 / 02期
关键词：
Glutaraldehyde crosslinking; immobilized inositol phosphate synthase; bound NAD; synthase; immobilized inositol phosphate; NAD; immobilization of bound;
D O I：
10.1007/BF02779242
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
myo-Inositol-1-phosphate synthase (EC 5.5.1.4) from rat testes, an NAD(+)-containing enzyme, which converts D-glucose 6-phosphate to 1L-myo-inositol 1-phosphate, could be immobilized together with its cofactor and bovine serum albumin by crosslinking with glutaraldehyde at pH 4.5. The enzyme bound to the gel showed a specific activity of 5.6% of that of the native enzyme, but the activity could be increased to 21% by pretreatment with urea.
引用
收藏
页码：85 / 90
页数：6
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