SYNTHESIS OF SIALYLLACTOSE FROM N-ACETYLNEURAMINIC ACID AND LACTOSE BY A NEURAMINIDASE FROM ARTHROBACTER-UREAFACIENS

被引：21
|
作者：
MARU, I
OHTA, Y
OKAMOTO, K
SUZUKI, S
KAKEHI, K
TSUKADA, Y
机构：
[1] NIPPON ZOKI PHARMACEUT CO LTD, INST BIOACT SCI, YASHIRO, HYOGO 67314, JAPAN
[2] KINKI UNIV, FAC PHARMACEUT SCI, OSAKA, OSAKA 577, JAPAN
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1992年 / 56卷 / 10期
关键词：
D O I：
10.1271/bbb.56.1557
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two sialyllactose isomers, NeuAcalpha2-->6Galbeta1-->4Glc and Galbeta1-->4(NeuAcalpha2-->6)Glc, were prepared by incubation of a concentrated solution of N-acetylneuraminic acid and lactose in the presence of a neuraminidase from Arthrobacter ureafaciens. Each sialyllactose was isolated by a combination of ion-exchange chromatography and high performance liquid chromatography. The structure of each sialyllactose was identified by mass spectrometry, nuclear magnetic resonance spectrometry, and enzymatic analysis.
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收藏
页码：1557 / 1561
页数：5
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