RAPID KINETICS OF DEPOLARIZATION-INDUCED CHANGES IN INTRASYNAPTOSOMAL CALCIUM CONCENTRATIONS

被引：10
|
作者：
TAREILUS, E [1 ]
BREER, H [1 ]
机构：
[1] UNIV STUTTGART HOHENHEIM,INST ZOOPHYSIOL,GARBENSTR 30,W-7000 STUTTGART 70,GERMANY
来源：
NEUROCHEMISTRY INTERNATIONAL | 1992年 / 20卷 / 03期
关键词：
D O I：
10.1016/0197-0186(92)90041-O
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The rapid kinetics of depolarization-evoked calcium influxes in isolated nerve terminals from rat cortex were monitored by stopped-flow spectrofluorimetry using specific indicators (Fluo-3, Indo-1). A very rapid increase in the intrasynaptosomal Ca2+-level was detected within the subsecond time range after depolarizing synaptosomes by mixing with physiological saline containing elevated K+-concentrations. About 15 mM [K+]o was determined as threshold concentration for inducing Ca2+-influx, which increased with higher concentration and saturated at [K+]o-concentrations of about 40 mM [K+]o.
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页码：275 / 279
页数：5
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