PROTEOLYTIC-ENZYMES IN HUMAN LEUKEMIC LYMPHOID-CELLS .1. ACTIVITIES OF DIPEPTIDYL AMINOPEPTIDASE-IV, PLASMINOGEN-ACTIVATOR, AND CATHEPSIN-B AND CATHEPSIN-L IN CELLS WITH DIFFERENT IMMUNOLOGICAL PHENOTYPE

被引：0

作者：

LOKSHINA, LA ^{[1
]}

BYLINKINA, VS ^{[1
]}

SAMOILOVA, RS ^{[1
]}

GUREEVA, TA ^{[1
]}

GOLUBEVA, NV ^{[1
]}

POLYANSKAYA, AM ^{[1
]}

机构：

[1] RUSSIAN ACAD MED SCI,HEMATOL RES CTR,INST HEMATOL & INTENS THERAPY,MOSCOW,RUSSIA

来源：

BIOCHEMISTRY-MOSCOW | 1993年 / 58卷 / 07期

关键词：

DIPEPTIDYL AMINOPEPTIDASE IV; PLASMINOGEN ACTIVATOR (UROKINASE TYPE); CATHEPSINS B AND L; LYMPHOID B- AND T-CELLS; ANTIGENS; DIFFERENTIATION; LYMPHOPROLIFERATIVE DISORDERS;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lymphoid cells from 20 patients with various forms of lymphoproliferative disorders were studied immunologically and biochemically. Lymphoid cells with different immunological phenotype differed in their activities of dipeptidyl aminopeptidase IV (DAP IV), plasminogen activator (urokinase type), and cathepsins B + L. The highest activity of these proteinases was found in lysates of leukemic T-cells, the differentiation in which corresponded to the initial stage of thymic maturing or to activated mature T-cells. Phenotypically mature T-helpers (T-h) and T-suppressors (T-s) exhibited much lower and virtually the same activity. The activity of proteinases did not differ significantly in lymphoid cells of B-lineage (from pre-B-lymphocytes to mature B-cells), but it was 2 to 3 times lower than in lymphoid cell progenitors. A regulated change in activity of certain proteinases is suggested to occur during differentiation of B- and T-cells. An increase in the activity of DAP IV and cathepsins B + L likely occurs upon activation of mature lymphoid B- and T-cells. No direct relationship between the activity of either of the examined proteinases and the expression of any studied antigen could be revealed.

引用

页码：781 / 788

页数：8