THE DETERMINATION OF 3-AMINO-5-ETHYL-6-METHYL-PYRIDIN-2-ONE IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

A method for the determination of 3-amino-5-ethyl-6-methyl-pyridin-2-one in human plasma is described. Buffered (pH 8) plasma samples are extracted with 7.5% 2-propanol in chloroform after the addition of an internal standard. The extracts are evaporated to dryness and reconstituted in mobile phase prior to analysis via HPLC with fluorescence detection (lambda(ex)=314 nm, lambda(em)=390 nm). The assay has been validated in the concentration range of 5-500 ng/ml when 1 ml samples are extracted. Application of the assay to the analysis of samples collected after oral dosing of a 2-pyridinone based specific HIV-1 reverse transcriptase inhibitor is demonstrated.