CYTOSKELETAL INTEGRITY IN INTERPHASE CELLS REQUIRES PROTEIN PHOSPHATASE-ACTIVITY

被引:155
|
作者
ERIKSSON, JE
BRAUTIGAN, DL
VALLEE, R
OLMSTED, J
FUJIKI, H
GOLDMAN, RD
机构
[1] BROWN UNIV,BIOCHEM SECT,PROVIDENCE,RI 02912
[2] WORCESTER FDN EXPTL BIOL INC,DEPT CELL BIOL,SHREWSBURY,MA 01545
[3] UNIV ROCHESTER,DEPT BIOL,ROCHESTER,NY 14627
[4] NATL CANC CTR,RES INST,DIV CANC PREVENT,CHUO KU,TOKYO 104,JAPAN
关键词
D O I
10.1073/pnas.89.22.11093
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Phosphorylation by protein kinases has been established as a key factor in the regulation of cytoskeletal structure. However, little is known about the role of protein phosphatases in cytoskeletal regulation. To assess the possible functions of protein phosphatases in this respect, we studied the effects of the phosphatase inhibitors calyculin A, okadaic acid, and dinophysistoxin 1 (35-methylokadaic acid) on BHK-21 fibroblasts. Within minutes of incubation with these inhibitors, changes are seen in the structural organization of intermediate filaments, followed by a loss of microtubules, as assayed by immunofluorescence. These changes in cytoskeletal structure are accompanied by a rapid and selective increase in vimentin phosphorylation on interphase-specific sites, and they are fully reversible after removal of calyculin A. The results indicate that there is a rapid phosphate turnover on cytoskeletal intermediate filaments and further suggest that protein phosphatases are essential for the maintenance and structural integrity of two major cytoskeletal components.
引用
收藏
页码:11093 / 11097
页数:5
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