2,3-BUTANEDIONE MONOXIME BLOCKADE OF CA2+ CURRENTS IN ADULT-RAT SYMPATHETIC NEURONS DOES NOT INVOLVE CHEMICAL PHOSPHATASE-ACTIVITY

被引：21
|
作者：
YU, Z
IKEDA, SR
机构：
[1] Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta
来源：
NEUROSCIENCE LETTERS | 1993年 / 155卷 / 01期
关键词：
BUTANEDIONE MONOXIME; CALCIUM CURRENT; CAMP; DEPHOSPHORYLATION; PATCH CLAMP; SYMPATHETIC NEURON;
D O I：
10.1016/0304-3940(93)90665-8
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Effects of the 'chemical phosphatase' 2,3-butanedione monoxide (BDM) on voltage-activated Ca2+ currents of adult rat superior cervical ganglion neurons were investigated using the whole-cell patch-clamp technique. BDM produced a rapid (<10 s), reversible and dose-dependent (IC50 = 18.3 mM) inhibition of Ca2+ currents. The action of BDM was not prevented by 1 mM 8-(4-chlorophenylthio)-cAMP or 50 muM isoproterenol. H-7, a non-specific protein kinase inhibitor at 200 muM, did not prevent the rapid recovery from BDM-induced inhibition. Our results suggest that BDM inhibition of Ca2+ currents in rat sympathetic neurons does not require a 'chemical phosphatase' activity.
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页码：24 / 28
页数：5
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