STUDY OF A LIPASE FROM CANDIDA-RUGOSA DIDDENS AND LODDER

被引：0
|
作者：
RIAUBLANC, A [1 ]
RATOMAHENINA, R [1 ]
GALZY, P [1 ]
机构：
[1] INRA,ENSA,CHAIRE MICROBIOL IND & GENET MICROORGANISMES,PL VIALA,F-34060 MONTPELLIER,FRANCE
来源：
FETT WISSENSCHAFT TECHNOLOGIE-FAT SCIENCE TECHNOLOGY | 1993年 / 95卷 / 04期
关键词：
D O I：
暂无
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Lipasic system of Candida rugosa (CBS 613) strain was studied. The enzyme was purified in one step by hydrophobic chromatography. The properties of this lipase were determined. It is an oligomeric enzyme composed of five identical monomers of 46 kg . mol-1. Its optimum reaction conditions are pH = 7 and temperature = 40-degrees-C. This enzyme presents a rapid thermal denaturation and then a more stable form. It is a cell-bound lipase which is induced by triacyl glycerols. This enzyme presents a high specificity for external positions on glycerol.
引用
收藏
页码：134 / 137
页数：4
相关论文
共 50 条
  • [1] DEACTIVATION OF CANDIDA-RUGOSA LIPASE
    SOHN, HS
    CHUNG, SS
    RHEE, JS
    BIOTECHNOLOGY LETTERS, 1987, 9 (02) : 117 - 122
  • [2] IMMOBILIZATION STABILIZATION OF LIPASE FROM CANDIDA-RUGOSA
    OTERO, C
    BALLESTEROS, A
    GUISAN, JM
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1988, 19 (02) : 163 - 175
  • [3] CRYSTALLIZATION AND CHARACTERIZATION OF A CANDIDA-RUGOSA LIPASE
    RUBIN, B
    CYGLER, M
    SMITH, P
    LI, YG
    HARRISON, D
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1992, 203 : 94 - BIOL
  • [4] EFFECT OF POLYVINYLALCOHOLS ON THE THERMOSTABILITY OF LIPASE FROM CANDIDA-RUGOSA
    BATTISTEL, E
    BIANCHI, D
    DACUNZO, A
    DEALTERIIS, E
    PARASCANDOLA, P
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1995, 50 (02) : 161 - 173
  • [5] LIPASE PRODUCTION BY CANDIDA-RUGOSA - FERMENTATION BEHAVIOR
    VALERO, F
    AYATS, F
    LOPEZSANTIN, J
    POCH, M
    BIOTECHNOLOGY LETTERS, 1988, 10 (10) : 741 - 744
  • [6] LOCALIZATION OF LIPASE GENES ON CANDIDA-RUGOSA CHROMOSOMES
    BROCCA, S
    GRANDORI, R
    BREVIARIO, D
    LOTTI, M
    CURRENT GENETICS, 1995, 28 (05) : 454 - 457
  • [7] MULTIPLE FORMS AND FUNCTIONS OF CANDIDA-RUGOSA LIPASE
    CHANG, RC
    CHOU, SJ
    SHAW, JF
    BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 1994, 19 : 93 - 97
  • [8] LIPASE PRODUCTION BY IMMOBILIZED CANDIDA-RUGOSA CELLS
    FERRER, P
    SOLA, C
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1992, 37 (06) : 737 - 741
  • [9] RAPID PURIFICATION OF 2 LIPASE ISOENZYMES FROM CANDIDA-RUGOSA
    RUA, ML
    BALLESTEROS, A
    BIOTECHNOLOGY TECHNIQUES, 1994, 8 (01) : 21 - 26
  • [10] 2 CONFORMATIONAL STATES OF CANDIDA-RUGOSA LIPASE
    GROCHULSKI, P
    LI, Y
    SCHRAG, JD
    CYGLER, M
    PROTEIN SCIENCE, 1994, 3 (01) : 82 - 91
12345