QUALITATIVE AND QUANTITATIVE DETECTION OF HIV-1 RNA BY NUCLEIC-ACID SEQUENCE-BASED AMPLIFICATION

被引：64

作者：

VANGEMEN, B

KIEVITS, T

NARA, P

HUISMAN, HG

JURRIAANS, S

GOUDSMIT, J

LENS, P

机构：

[1] BLOOD TRANSFUS SERV,CENT LAB,AMSTERDAM,NETHERLANDS

[2] ACAD MED CENTRUM AMSTERDAM,AMSTERDAM,NETHERLANDS

[3] NCI,TUMOR CELL BIOL LAB,VIRUS BIOL SECT,FREDERICK,MD

来源：

AIDS | 1993年 / 7卷

关键词：

NUCLEIC ACID SEQUENCE-BASED AMPLIFICATION; HIV-1; RNA; AMPLIFICATION; QUANTIFICATION; MUTATION DETECTION;

D O I：

10.1097/00002030-199311002-00020

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Aim: To develop a method to detect HIV-1 viral RNA by amplifying a specific nucleic acid sequence. Method: The nucleic acid sequence-based amplification (NASBA) method uses the simultaneous activity of avian myeloblastosis virus reverse transcriptase, T7 RNA polymerase and RNase H to amplify a specific nucleic acid target sequence. Validation: An in vitro cultured HIV-1 stock solution was used to validate the NASBA method and determine the variation in RNA measurement. Conclusion: Although NASBA is theoretically capable of specific amplification of RNA or DNA, it is most suitable for amplification of RNA, and therefore for detection of HIV-1 viral RNA.

引用

页码：S107 / S110

页数：4

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