PLANT-TISSUE CULTURE USING BRASSICA SEEDLINGS

A tissue culture practical is described which uses vegetable Brassica seedlings germinated in-vitro and cut up into root, hypocotyl, and cotyledon explants. These are then cultured on media containing different levels of phytohormones which induce shoot, root, and callus production in as little as Four to six weeks. The practical is simple and easy to use and does not require the use of a lamina flow cabinet. Further developments from the basic schedule are suggested.