DIFFERENTIAL RECOGNITION OF A SET OF O-ACETYLATED GANGLIOSIDES BY MONOCLONAL-ANTIBODIES RB13-2, D1.1, AND JONES DURING RAT-BRAIN DEVELOPMENT

被引：27

作者：

REINHARDTMAELICKE, S ^{[1
]}

CLEEVES, V ^{[1
]}

KINDLERROHRBORN, A ^{[1
]}

RAJEWSKY, MF ^{[1
]}

机构：

[1] UNIV ESSEN GESAMTHSCH,INST ZELLBIOL TUMORFORSCH,HUFELANDSTR 55,W-4300 ESSEN 1,GERMANY

来源：

DEVELOPMENTAL BRAIN RESEARCH | 1990年 / 51卷 / 02期

关键词：

Brain development; Ganglioside; Immunoblot; Indirect immunofluorescence; Monoclonal antibody; Neural precursor cell;

D O I：

10.1016/0165-3806(90)90286-8

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Monoclonal antibodies (Mab) RB13-2, JONES, and D1.1 all recognize gangliosides expressed on the surface of prenatal rat brain cells. As shown by high-performance thin-layer chromatography (HPTLC) immunoblotting of enriched rat brain gangliosides from different stages of development, all these Mabs bind to O-acetylated GD3 and (weakly) to an as yet unidentified double-band. Both Mab RB13-2 and Mab D1.1 recognize two additional minor gangliosides. Immunofluorescence analyses of frozen serial sections of prenatal rat brain show that, in addition to the cell subpopulations stained with all three antibodies, Mab RB13-2 and Mab D1.1 recognize further subpopulations of neural cells. These additional Mab JONES-negative cells apparently express minor ganglioside(s) identified in the immunoblots. © 1990.

引用

页码：279 / 282

页数：4