HYDROXYL RADICAL-SCAVENGING PROPERTY OF INDOMETHACIN

被引：18
|
作者：
PRASAD, K
LAXDAL, VA
机构：
[1] UNIV SASKATCHEWAN, COLL MED, DEPT PATHOL, SASKATOON S7N 0W0, SK, CANADA
[2] ROYAL UNIV HOSP, SASKATOON, SK, CANADA
来源：
MOLECULAR AND CELLULAR BIOCHEMISTRY | 1994年 / 136卷 / 02期
关键词：
HYDROXYL RADICALS; INDOMETHACIN; 2,3-DIHYDROXYBENZOIC ACID; 2,5-DIHYDROXYBENZOIC ACID; PHOTOLYSIS OF H2O2; HYDROXYL RADICAL SCAVENGER;
D O I：
10.1007/BF00926074
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The ability of indomethacin to scavenge hydroxyl radical (.OH) using high pressure liquid chromatography (HPLC) was investigated. .OH radical was generated by photolysis of H2O2 (1.5-1 0 mmoles/l) with UV light and was trapped with salicyclic acid (500 nmoles). H2O2 produced .OH in a concentration-dependent manner as estimated by .OH adduct products 2,3- and 2,5-dihydroxybenzoic acid (DHBA). Indomethacin in increasing concentrations (5-600 mu moles/L) produced increasing inhibition of generation of 2,3-DHBA (7-67%) and of 2,5-DHBA (7-77%). The results indicate that indomethacin scavenges .OH in a concentration-dependent manner.
引用
收藏
页码：139 / 144
页数：6
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