LOCALIZATION OF THE STEROID-BINDING SITE OF THE HUMAN SEX STEROID-BINDING PROTEIN OF PLASMA (SBP OR SHBG) BY SITE-DIRECTED MUTAGENESIS

被引：20
|
作者：
SUI, LM
CHEUNG, AWC
NAMKUNG, PC
PETRA, PH
机构：
[1] UNIV WASHINGTON,DEPT OBSTET & GYNECOL,SEATTLE,WA 98195
[2] UNIV WASHINGTON,DEPT BIOCHEM,SEATTLE,WA 98195
来源：
FEBS LETTERS | 1992年 / 310卷 / 02期
关键词：
SEX STEROID-BINDING PROTEIN; SBP; SEX HORMONE BINDING GLOBULIN; SHBG; MAMMALIAN EXPRESSION; SITE-DIRECTED MUTAGENESIS;
D O I：
10.1016/0014-5793(92)81309-A
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The amino-terminal region of the human sex steroid-binding protein of plasma (SBP or SHBG) containing K134 and M139 was found to represent part of the steroid-binding site. This was accomplished by constructing and expressing site-directed mutants having the following replacements: M139L, M139K, M139S, K134A, H235S, and Y57F. The results indicated that M139L and H235S were fully-active. K134A and Y57F were 50 and 67% active. M139K was 7% active, and M139S was inactive. These results support affinity-labeling data indicating that both K134 and M139 are located in or near the site, and suggest that Y57 may play a role in steroid binding. The fully active H235S mutant reveals that H235 is not involved in the steroid-binding process.
引用
收藏
页码：115 / 118
页数：4
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