HUMAN RIBOSOMAL-PROTEIN L37A - CLONING OF THE CDNA AND ANALYSIS OF DIFFERENTIAL GENE-EXPRESSION IN TISSUES AND CELL-LINES

被引：16
|
作者：
SAHA, DP
TIRUMALAI, PS
SCALA, LA
HOWELLS, RD
机构：
[1] UMDNJ,NEW JERSEY MED SCH,DEPT BIOCHEM & MOLEC BIOL,NEWARK,NJ 07103
[2] UNIV MED & DENT NEW JERSEY GRAD SCH BIOMED SCI,NEWARK,NJ 07103
来源：
GENE | 1993年 / 132卷 / 02期
关键词：
LARGE RIBOSOMAL SUBUNIT PROTEIN; MOLECULAR CLONING; NEUROBLASTOMA CELLS; POLYMERASE CHAIN REACTION; INTRON-LESS; ZINC FINGER;
D O I：
10.1016/0378-1119(93)90209-L
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A cDNA corresponding to human ribosomal protein L37a (hL37a) was obtained by screening a SHSY5Y neuroblastoma library. The full-length cDNA contained 366 nucleotides (nt) in addition to a poly(A) tail. The pyrimidine-rich sequence, CTTTCT, that is common to many ribosomal protein-encoding cDNAs was present at the 5' terminus. The nt sequence displayed 85% identity with rat L37 alpha (rL37 alpha) cDNA. The predicted protein contains 92 amino acids with a M(r) of 10 277, is highly basic, and has 100% sequence identity with rL37a. A putative zinc-finger domain is present in the central region of the protein. Human lymphocytes and several human cell lines express hL37 alpha mRNA at significantly higher levels than the rat cell lines and rat tissues tested. The hL37 alpha gene does not contain introns.
引用
收藏
页码：285 / 289
页数：5
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