CELLULASE PRODUCTION BY NEUROSPORA-CRASSA - PURIFICATION AND CHARACTERIZATION OF CELLULOLYTIC ENZYMES

被引：29
|
作者：
YAZDI, MT
RADFORD, A
KEEN, JN
WOODWARD, JR
机构：
[1] UNIV LEEDS,BIOTECHNOL UNIT,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND
[2] UNIV LEEDS,DEPT GENET,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND
[3] UNIV LEEDS,DEPT BIOCHEM,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 1990年 / 12卷 / 02期
关键词：
Endoglucanase; exoglucanase; purification; β-glucosidase;
D O I：
10.1016/0141-0229(90)90084-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In studies on cellulase production by the cell-1 mutant of Neurospora crassa, eight enzymes (three exoglucanases, four endoglucanases, and one β-glucosidase) were identified and characterized by gel filtration, ion exchange chromatography, and chromatofocusing. After purification, each of the proteins ran as a single band in polyacrylamide gel electrophoresis, using both native and denaturing gels. The molecular weights of the proteins were found to be between 70,000 and 22,000 daltons, and all were glycosylated, with carbohydrate contents ranging between 5.6% and 36%. © 1990.
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页码：120 / 123
页数：4
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