MONITORING THE SURFACE-TENSION OF LIPID-MEMBRANES BY A BUBBLE METHOD

被引：4
|
作者：
RING, A
机构：
[1] Department of Physiology and Medical Biophysics, Biomedical Centre, Uppsala, S-75123
来源：
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1992年 / 420卷 / 3-4期
关键词：
LIPID MEMBRANE; SURFACE TENSION; ION CHANNELS;
D O I：
10.1007/BF00374457
中图分类号：
Q4 [生理学];
学科分类号：
071003 ;
摘要：
A new method for the determination of lipid membrane surface tension was developed. Advantages of this method are that it allows for multiple measurements on a single membrane, is fast and direct not requiring empirical corrections, may be applied for dynamical surface-tension measurements and may be used with thin films and asymmetrical electrolytes. The pressure and radius of a bubble are measured. A piezoresistive sensor is used to minimize the transducer compliance. By moulding the sensor to a brass plate a resolution of 0.025 mm H2O (0.25 Pa) is obtained. The bubble is filmed using a videocamera and the radius of the bubble determined with the aid of a microcomputer. Data for monoolein/hexadecane in potassium chloride solutions and a cooling curve are presented and compared with previous results.
引用
收藏
页码：264 / 268
页数：5
相关论文
共 50 条
12345