GLUTENIN LOCI VARIABILITY OF CROATIAN WHEAT GERMPLASM

Glutenins loci were used for variability estimation in 50 varieties of hexaploid winter wheat originated from Croatian breeding centres. Polyacrylamide gel electrophoresis (PAGE) in presence of sodium dodecyl sulphate (SDS) was used for determination of high molecular weight glutenins (HMW-GS). Number of allels per loci ranged from 3 at Glu-A1 to 5 at Glu-B1 and Glu-D1, the average number of allels was 4.33. The highest genetic diversity was found at loci Glu-B1 (H-e = 0.687). The most frequent subunit at loci Glu-Al was 2* (56%). At loci Glu-B1 the most common combination of subunits was 7+8 with 40%, and at loci Glu-D1 5+10 with 68%. The study also defines high quality varieties with largest number of Glu-scores. The results attained from this study allow further development of specific breeding programs for winter wheat quality improvement and improvers creation.