VOLTAGE-DEPENDENT AND TIME-DEPENDENT INHIBITION OF NEURONAL CALCIUM CHANNELS BY A GTP-BINDING PROTEIN IN A MAMMALIAN-CELL LINE

1. Inhibitory modulation of the three Ca2+ channel current components by neurotransmitters was studied using the whole-cell patch-clamp method in a mammalian cell line, NG108-15. 2. In cells differentiated with dibutyryl cyclic AMP, both the low-voltage-activated current (I(LVA)) and omega-CgTX-sensitive high-voltage-activated current (I(omega-CgTX)) could be inhibited by [D-phen2, D-phen5]enkephalin, acetylcholine and noradrenaline. In contrast, differentiation with prostaglandin E1 and theophylline eliminated the agonist-induced inhibition of I(LVA), but enhanced that of I(omega-CgTX). The DHP-sensitive high-voltage-activated current was unaffected by the transmitters in most of the cells. 3. The inhibition was prevented by pre-treatment of cells with pertussis toxin, suggesting involvement of a G-protein. Long treatment of the cells with phorbol ester did not prevent the inhibition. 4. The inhibition was always partial: the maximal inhibition was 40% for I(LVA) and 70% for I(omega-CgTX). 5. The inhibition of I(LVA) and I(omega-CgTX) was relieved during depolarization. Half-maximal relief of inhibition of I(omega-CgTX) was attained at 0 mV, irrespective of agonist concentration. 6. The kinetics of removal and re-establishment of inhibition were voltage dependent. Both processes were single exponentials and had identical time constants at a given membrane potential. Time constants were 124 ms at -40 mV, 160 ms at 0 mV and 8 ms at 60 mV, at any agonist concentration. 7. Time courses of tail currents were unaltered by the inhibition. 8. The inhibition of the omega-CgTX-sensitive Ca2+ channel can be described as a shift in gating modes; with an additional voltage-dependent gating state activated by the agonists. The voltage-dependent properties of this modulation allow inhibition of Ca2+ channel to be overcome by high-frequency trains of action potentials.