FUCOSE CONTENT OF KERATAN SULFATES FROM BOVINE ARTICULAR-CARTILAGE

被引：29
|
作者：
TAI, GH
BROWN, GM
MORRIS, HG
HUCKERBY, TN
NIEDUSZYNSKI, IA
机构：
[1] UNIV LANCASTER, INST ENVIRONM & BIOL SCI, DIV BIOL SCI, LANCASTER LA1 4YQ, ENGLAND
[2] UNIV LANCASTER, DEPT CHEM, LANCASTER LA1 4YA, ENGLAND
来源：
BIOCHEMICAL JOURNAL | 1991年 / 273卷
关键词：
D O I：
10.1042/bj2730307
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6-8-year-old animals). Nine keratan sulphate fractions of increasing molecular weight were prepared by gel-permeation chromatography on a calibrated column of TSK 30 XL. The samples were analysed for fucose and galactose contents (% by wt. of keratan sulphate) and fucose/gatactose ratio. The fucose content increased with molecular size, but the galactose content remained constant. It was concluded that the alpha(1 --> 3)-linked fucose [Thornton, Morris, Cockin, Huckerby, Nieduszynski, Carlstedt, Hardingham & Ratcliffe (1989) Biochem. J. 260, 277-282] was located within the poly-N-acetyl-lactosamine repeat sequence of articular-cartilage keratan sulphate.
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页码：307 / 310
页数：4
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