COMPARISON OF 2 METHODS FOR CALLUS-CULTURE AND PLANT-REGENERATION IN WHEAT (TRITICUM-AESTIVUM)

被引：0
|
作者：
MOHMAND, AS [1 ]
NABORS, MW [1 ]
机构：
[1] COLORADO STATE UNIV,DEPT BIOL BOT,FT COLLINS,CO 80523
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 1991年 / 26卷 / 03期
关键词：
AGAR MEDIUM; CALLUS SIZE; EMBRYOGENIC CALLUS; FILTER PAPER BRIDGES; POLYETHYLENE GLYCOL;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Callus growth and development involve a complex relationship between the explants used to initiate callus, the constituents of the medium and the environmental conditions during culturing. Use of high molecular weight osmotica such as polyethylene glycol (PEG-4000) results in non-solidification of agar medium used for culturing and selection. Thus, a new filter paper bridge technique was compared with the existing agar medium for callus initiation, multiplication, and plant regeneration of wheat. The yield of both total and embryogenic callus was doubled and significantly higher number of regenerants was obtained on filter paper bridges compared to agar medium.
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页码：185 / 187
页数：3
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