A NEW SHUTTLE VECTOR FOR BACILLUS-STEAROTHERMOPHILUS AND ESCHERICHIA-COLI

被引：8
|
作者：
NAKAYAMA, N
NARUMI, I
NAKAMOTO, S
KIHARA, H
机构：
[1] UTSUNOMIYA UNIV,DEPT AGR CHEM,UTSUNOMIYA,TOCHIGI 321,JAPAN
[2] JICHI MED SCH,SCH NURSING,MINAMI KAWACHI,TOCHIGI 32904,JAPAN
来源：
BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 08期
关键词：
D O I：
10.1007/BF01021636
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A new shuttle vector was constructed by inserting a 3.1 kbp-DNA fragment from thermophilic Bacillus sp. plasmid pIH41 into Escherichia coli plasmid pUC18. The resultant hybrid replicates in both E. coli and B. stearothermophilus. This vector has ten unique restriction sites within a part of lacZ gene. Insertion of foreign DNA into these sites can be readily detected by a coloration method.
引用
收藏
页码：649 / 652
页数：4
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