N-TERMINAL AMINO-ACID-SEQUENCE OF A 28-KDA MAJOR SERUM HIGH-DENSITY-LIPOPROTEIN OF THE RAINBOW-TROUT ONCORHYNCHUS-MYKISS

被引：0
|
作者：
MURATA, M
SUGIMOTO, C
KODAMA, H
ONUMA, M
机构：
[1] UNIV OSAKA PREFECTURE, COLL AGR, DEPT VET SCI, VET IMMUNOL LAB, SAKAI, OSAKA 593, JAPAN
[2] HOKKAIDO UNIV, FAC VET MED, DEPT EPIZOOTIOL, SAPPORO, HOKKAIDO 060, JAPAN
来源：
JAPANESE JOURNAL OF VETERINARY RESEARCH | 1994年 / 42卷 / 02期
关键词：
HIGH-DENSITY SERUM LIPOPROTEIN; RAINBOW TROUT (ONCORHYNCHUS-MYKISS); SALMONID;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
As there are few data on primary structures of fish apolipoproteins (apo), we determined the N-terminal sequence of a 28 kDa major serum protein of rainbow trout, a possible homologue of apoAI protein in higher vertebrates. Rainbow trout serum was separated by two-dimensional electrophoresis. A spot of the 28 kDa protein (pl 5.45) was cut out and directly applied to a protein sequencer. We succeded in sequencing 30 amino acids from the N-terminal. The sequence of the 28 kDa protein shared a high similarity with that of apoAI of Atlantic salmon (Salmo salar). These results showed that the 28 kDa protein is apoAI of rainbow trout.
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页码：89 / 94
页数：6
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