A METHOD FOR ENZYME-HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL STAINING OF LARGE FROZEN SPECIMENS

被引：4
|
作者：
HOLMBOM, B [1 ]
LINDSTROM, M [1 ]
NASLUND, U [1 ]
THORNELL, LE [1 ]
机构：
[1] UMEA UNIV,DEPT INTERNAL MED,S-90187 UMEA,SWEDEN
来源：
HISTOCHEMISTRY | 1991年 / 95卷 / 05期
关键词：
D O I：
10.1007/BF00315739
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A method for large specimen cryosectioning is described. Specimens of pig heart ventricles were lightly fixed by microwave irradiation, embedded in 10% gelatin, frozen in hexan chilled with dry ice, and sectioned using an LKB 2250 PMW cryomicrotome. The sections were collected on transparent film and transferred onto glass slides. Standard histological, enzyme-and immuno-histochemical staining techniques were used. The present method allowed cryosectioning with sections of good quality which could be used for enzyme-and immuno-staining. For studies of experimental myocardial infarction, staining for phosphorylase and Periodic Acid Schiff outlined the ischemic area and antibodies against plasma fibronectin and fibrinogen delineated the infarcted myocardium.
引用
收藏
页码：441 / 447
页数：7
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