A DOMINANT POSITIVE AND NEGATIVE SELECTABLE GENE FOR USE IN MAMMALIAN-CELLS

被引：45
|
作者：
SCHWARTZ, F
MAEDA, N
SMITHIES, O
HICKEY, R
EDELMANN, W
SKOULTCHI, A
KUCHERLAPATI, R
机构：
[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT MOLEC GENET,BRONX,NY 10461
[2] UNIV N CAROLINA,DEPT PATHOL,CHAPEL HILL,NC 27599
[3] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT CELL BIOL,BRONX,NY 10461
[4] UNIV ILLINOIS,COLL MED,DEPT GENET,CHICAGO,IL 60612
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 23期
关键词：
GENE FUSION; HERPES SIMPLEX VIRUS THYMIDINE KINASE; BACTERIAL NEOMYCIN PHOSPHOTRANSFERASE;
D O I：
10.1073/pnas.88.23.10416
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We have constructed three different fusion genes containing the herpes simplex virus thymidine kinase (HSV tk) and the bacterial neomycin phosphotransferase (neo) genes. All three fusion genes utilize the HSV tk promoter but differ at the junction of their components. We have determined if the fusion genes are bifunctional by introducing them into mammalian cells and testing for function of the individual components. One of the fusion genes, TNFUS 69, produced a bicistronic message and a fusion protein that has TK and NEO protein functions. This and other fusion genes of a similar nature could serve as dominant positive and negative selectable markers in mammalian cells.
引用
收藏
页码：10416 / 10420
页数：5
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