CONTRIBUTION OF AD4BP, A STEROIDOGENIC CELL-SPECIFIC TRANSCRIPTION FACTOR, TO REGULATION OF THE HUMAN CYP11A AND BOVINE CYP11B GENES THROUGH THEIR DISTAL PROMOTERS

被引:54
|
作者
TAKAYAMA, K [1 ]
MOROHASHI, K [1 ]
HONDA, S [1 ]
HARA, N [1 ]
OMURA, T [1 ]
机构
[1] KYUSHU UNIV,FAC MED,CHEST DIS RES INST,HIGASHI KU,FUKUOKA 812,JAPAN
来源
JOURNAL OF BIOCHEMISTRY | 1994年 / 116卷 / 01期
关键词
AD4 BINDING PROTEIN; CYP11A GENE; CYP11B GENE; DISTAL PROMOTER; TRANSCRIPTIONAL REGULATION;
D O I
10.1093/oxfordjournals.jbchem.a124493
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We analyzed the upstream regions of the human CYP11A and bovine CYP11B genes, and identified a distal promoter in each gene. The distal promoters are located at -1.8 to -1.5 kb in the upstream region of the CYP11A gene and -1.5 to -1.1 kb in the upstream region of the CYP11B gene. Transient transfection of CAT plasmid carrying each of the two distal promoters indicated that the regions had a transcriptional activating function and that the function was stimulated by cAMP. The basal and cAMP-stimulated transcriptional activities were detected only in steroidogenic cells. On structural analyses of the regions, we identified two Ad4 sites and a cAMP responsive element in the distal promoter of CYP11A, and two Ad4 sites and one NF-IL6 binding site in the distal promoter of CYP11B. The presence of an Ad4 site in common suggests its major contribution to the transcriptional activation, We also investigated the functional interactions between the distal promoters and basal promoters of both genes. Interestingly, the two distal promoters showed different requirements as to the basal promoter.
引用
收藏
页码:193 / 203
页数:11
相关论文
共 3 条
  • [1] ACTIVATION OF CYP11A AND CYP11B GENE PROMOTERS BY THE STEROIDOGENIC CELL-SPECIFIC TRANSCRIPTION FACTOR, AD4BP
    MOROHASHI, K
    ZANGER, UM
    HONDA, S
    HARA, M
    WATERMAN, MR
    OMURA, T
    MOLECULAR ENDOCRINOLOGY, 1993, 7 (09) : 1196 - 1204
  • [2] FUNCTION AND DISTRIBUTION OF A STEROIDOGENIC CELL-SPECIFIC TRANSCRIPTION FACTOR, AD4BP
    MOROHASHI, K
    HATANO, O
    NOMURA, M
    TAKAYAMA, K
    HARA, M
    YOSHII, H
    TAKAKUSU, A
    OMURA, T
    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1995, 53 (1-6): : 81 - 88
  • [3] Regulation of Kruppel-like factor 4, 9, and 13 genes and the steroidogenic genes LDLR, StAR, and CYP11A in ovarian granulosa cells
    Natesampillai, Sekar
    Kerkvliet, Jason
    Leung, Peter C. K.
    Veldhuis, Johannes D.
    AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2008, 294 (02): : E385 - E391