Effect of concentration, pressure, and cut-off size on removing phospholipase A(2) in bee venom by ultrafiltration

被引：0

作者：

Lee, Ji-yeon ^{[1
]}

Kim, Il Kwang ^{[2
]}

Lee, Jong Soo ^{[3
]}

Kim, Eui Kyung ^{[4
]}

Kim, Choul-Goo ^{[1
]}

机构：

[1] Hanyang Univ, Chungjin Biotech, 1271 Sa3 Dong, Ansan 426791, Gyeonggi Do, South Korea

[2] Wonkwang Univ, N CosmeCeut Co Ltd, Iksan Si 570749, Jeollabuk Do, South Korea

[3] Chungnam Natl Univ, Coll Vet Med, Deajeon 305764, South Korea

[4] Gyeongsang Natl Univ, Coll Vet Med, Dept Pharmacol & Toxicol, Jinju 660701, South Korea

来源：

ANALYTICAL SCIENCE AND TECHNOLOGY | 2014年 / 27卷 / 06期

关键词：

honey; bee venom; phospholipase A(2); ultrafiltration;

D O I：

10.5806/AST.2014.27.6.277

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The effects of concentration, pressure, and molecular sige on removing allergenic substance (phospholipase A(2)) in bee venom by ultrafiltration were investigated. The membrane pore sizes were selected based on the molecular weight of the main compounds. The conditions of concentration and pressure were selected randomly. As results, we obtained the optimum condition (1 mg/mL, 20 psi, 10,000 dalton) for removing PLA(2) at constant concentration of melittin and apamin and confirmed the separation results by HPLC and SDS-PAGE.

引用

页码：277 / 283

页数：7

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