THE CHEMICAL-ENZYMATIC SYNTHESIS OF THE TRANSLATIONAL ENHANCER OF THE PHAGE-T7 GENE-10 AND FUNCTION OF ITS STRUCTURAL ELEMENTS

被引：0

作者：

GUREVICH, AI ^{[1
]}

SCAPTSOVA, NV ^{[1
]}

LUTSENKO, SV ^{[1
]}

SMIRNOV, VA ^{[1
]}

KURKIN, AN ^{[1
]}

AZHAYEV, AV ^{[1
]}

机构：

[1] ALL UNION BIOTECHNOL RES INST,MOSCOW,USSR

来源：

BIOORGANICHESKAYA KHIMIYA | 1991年 / 17卷 / 05期

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摘要：

The translational enhancer (TREN) sequence of the phage T7 gene 10 (in full and also its proximal or distal parts) have been obtained by chemical-enzymatic synthesis and cloned into the plasmids immediately before the human interleukin 3 (hIL3) artificial gene. Expression levels of the hIL3 gene in E. coli in these constructions show that the region controlling the specific activity is placed in distal part of TREN more than 40 nucleotides upstream from the initiation codon.

引用

页码：647 / 652

页数：6

共 8 条

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BIOORGANICHESKAYA KHIMIYA, 1982, 8 (11): : 1501 - 1515
[5] TAC PROMOTER VECTORS INCORPORATING THE BACTERIOPHAGE-T7 GENE-10 TRANSLATIONAL ENHANCER SEQUENCE FOR IMPROVED EXPRESSION OF CLONED GENES IN ESCHERICHIA-COLI
LEHMEIER, B
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JOURNAL OF BIOTECHNOLOGY, 1992, 23 (02) : 153 - 165
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JOURNAL OF VIROLOGY, 1977, 22 (03) : 694 - 701
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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1970, 65 (01) : 242 - +
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