HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF HISTAMINE N-METHYLTRANSFERASE ACTIVITY

被引：21
|
作者：
FUKUDA, H
YAMATODANI, A
IMAMURA, I
MAEYAMA, K
WATANABE, T
WADA, H
机构：
[1] OSAKA UNIV,FAC MED,DEPT PHARMACOL 2,NAKANOSHIMA 4-3-57,KITA KU,OSAKA 530,JAPAN
[2] TOHOKU UNIV,SCH MED,DEPT PHARMACOL 1,AOBA KU,SENDAI,MIYAGI 980,JAPAN
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 567卷 / 02期
关键词：
D O I：
10.1016/0378-4347(91)80152-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method for the determination of histamine N-methyltransferase (HMT) activity by high-performance liquid chromatography based on post-column derivatization with o-phthalaldehyde is described. The determination involves the separation of the substrate, histamine, from its product, N-tau-methylhistamine, using a weak cation exchanger, followed by on-line derivatization of these imidazoleamines with omicron-phthalaldehyde and their detection and quantitation with a fluorimetric detector. This assay method is suitable for the measurement of HMT activity during enzyme purification.
引用
收藏
页码：459 / 464
页数：6
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