FORMATION OF SUBVIRAL PARTICLES BY INVITRO TRANSLATION OF SUBGENOMIC POLIOVIRUS RNAS

被引：8
|
作者：
JORE, JPM [1 ]
VELDHUISEN, G [1 ]
POUWELS, PH [1 ]
BOEYE, A [1 ]
VRIJSEN, R [1 ]
ROMBAUT, B [1 ]
机构：
[1] VRIJE UNIV BRUSSELS,DEPT MICROBIOL & HYG,B-1090 BRUSSELS,BELGIUM
来源：
JOURNAL OF GENERAL VIROLOGY | 1991年 / 72卷
关键词：
D O I：
10.1099/0022-1317-72-11-2721
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Rabbit reticulocyte lysates were programmed with either RNA extracted from purified poliovirus or a mixture of mRNAs encoding the capsid precursor, P1, and proteinase 3CD. In both cases, 14S subunits were formed at 30-degrees-C and empty capsids at 37-degrees-C. Both the 14S subunits and empty capsids had the expected polypeptide composition and neutralization epitopes. It is concluded that the proteinase 3CD gene is the only viral genetic information needed for the correct processing of P1 and the formation of 14S subunits, and their assembly into antigenically correct empty capsids.
引用
收藏
页码：2721 / 2726
页数：6
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