IDENTIFICATION OF INOCULANT STRAINS AND NATURALIZED POPULATIONS OF RHIZOBIUM-LEGUMINOSARUM BV TRIFOLII USING COMPLEMENTARY METHODOLOGIES

被引：1
|
作者：
FABIANO, E
ARIAS, A
机构：
[1] Division of Biochemistry, Instituto de Investigaciones Biologicas Clemente Estable (IIBCE), Montevideo
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1990年 / 6卷 / 02期
关键词：
D O I：
10.1007/BF01200931
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Introduced clovers (Trifolium repens and Trifolium subterraneum) fall to persist more than two years in Uruguayan improved pastures. Three strains of naturalized Rhizobium leguminosarum bv trifolii, isolated from persistent clover pastures inoculated several years ago, and two commercial inoculant strains have been identified. Serological techniques (enzyme-linked immunosorbent assay-ELISA) and total cell protein pattern have been selected as sensitive and reliable methods for strain identification. Indirect ELISA was suitable for detecting rhizobia in cultures or in nodules. Two of the naturalized strains had distinct protein profiles but they could not be differentiated by the immunological method with a polyclonal antibody. © 1990 Rapid Communications of Oxford Ltd.
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页码：121 / 126
页数：6
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