Detection of some Resistance genes in Klebsiella Pneumoniae and Proteus Mirabilis isolated from nosocomial infection in Baghdad hospital

Detection of encoded genes for some of the bata-lactame enzymes of Klebsiella pneumoniae and Proteus mirabilis isolated from patients in Baghdad hospitals using morphological and molecular methods. The study included the collection of two hundred and fifty clinical samples from patients infected with Enterobacteriaceae (128 males and 122 females), the clinical sample collected from (Urine, septum, wounds, burns, pulmonary fluids, ear swabs). The samples were identified by morphological methods and chemical tests, as well as for diagnosis using the VITEK2 system. The study revealed that Klebsiella spp (19.2%) and Proteus spp (10%). Sensitivity test of isolates sample was made by using disc diffusion method. Using Polymerase Chain Reaction (PCR) and Gel electrophoreses technique with the use of specific primers based on the World Bank Gene, six pathgenic genes were detected from beta-lactamas-resistant gene group (OXA-2and-20, OXA1-5and- KCP, OXA1, TEM, SHV). The results of isolating and diagnosing genes showed that K. pneumoniae and P.mirabilis had at least one of the resistance genes.