A MOUSE ERYTHROLEUKEMIA CELL-LINE POSSESSING FRIEND SPLEEN FOCUS-FORMING VIRUS GP55 TRANSGENE AND TEMPERATURE-SENSITIVE MUTANT P53 GENE

Two different erythroleukemia cell lines have been established from the splenic lesions of transgenic mice possessing the Friend spleen focus-forming virus (F-SFFV) gp55 gene, One showed a near-diploid karyotype and a temperature-sensitive (ts) p53 mutation, and the other, a hyper-triploid karyotype with double p53 mutations found by single-strand conformation polymorphism (SSCP) analysis. The cell lines both retained No,ll chromosomes on which p53 genes are localized, Another p53 allele in the cell line with the ts-p53 mutation appeared intact in the SSCP analysis of the genomic exon 5, The cells with the ts-mutant p53 gene showed no apparent change with temperature shift in their growth or dimethylsulfoxide-induced differentiation, although the wild-type p53 gene on the other allele was not expressing, This ts-p53(Val-135) gene made p53-deficient fibroblasts anchorage independent at 37 degrees C but not at 32 degrees C, This non-virus-producing, mouse erythroleukemia cell line will be useful for the study of mutated p53 function during the induction of erythrodifferentiation or apoptotic change.