METABOLIC-ACTIVATION OF M-PHENYLENEDIAMINE TO PRODUCTS MUTAGENIC IN SALMONELLA-TYPHIMURIUM BY MEDIUM ISOLATED FROM TOBACCO SUSPENSION CELL-CULTURES

被引：15

作者：

GICHNER, T ^{[1
]}

STAVREVA, DA ^{[1
]}

CEROVSKA, N ^{[1
]}

WAGNER, ED ^{[1
]}

PLEWA, MJ ^{[1
]}

机构：

[1] UNIV ILLINOIS, INST ENVIRONM STUDIES, URBANA, IL 61801 USA

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1995年 / 331卷 / 01期

关键词：

PLANT ACTIVATION; PROMUTAGENIC META-PHENYLENEDIAMINE; O-ACETYLTRANSFERASE; TOBACCO SUSPENSION CELLS; PEROXIDASE;

D O I：

10.1016/0027-5107(95)00058-Q

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Both tobacco cells in suspension and the medium recovered from the suspension cultures (TX1MX) activated the aromatic amine m-phenylenediamine (m-PDA) into a product that was mutagenic in Salmonella typhimurium TA98 and YG1024. Medium recovered from stationary-phase tobacco cell cultures exhibited the highest level of m-PDA activation. No cytochrome P-450 was detected in the activating medium. A high molecular weight matrix having the highest m-PDA activating capacity and associated with a substantial fraction of the total peroxidase activity was isolated by Centricon-100 ultrafiltration of TX1MX. The data suggest that the peroxidases present in the recovered cell culture medium or in the high molecular weight matrix are responsible for the plant activation of m-PDA.

引用

页码：127 / 132

页数：6

共 6 条

[1] Characterization of a macromolecular matrix isolated from tobacco suspension cell cultures and its role in the activation of promutagenic m-phenylenediamine
Stavreva, DA
Wagner, ED
Plewa, MJ
Gichner, T
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1997, 379 (02) : 191 - 199
[2] PLANT ACTIVATION OF M-PHENYLENEDIAMINE BY TOBACCO, COTTON, AND CARROT CELL-SUSPENSION CULTURES
LHOTKA, MA
PLEWA, MJ
GENTILE, JM
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 1987, 10 (01) : 79 - 88
[3] GENOTOXICITY OF M-PHENYLENEDIAMINE AND 2-AMINOFLUORENE IN SALMONELLA-TYPHIMURIUM AND HUMAN-LYMPHOCYTES WITH AND WITHOUT PLANT ACTIVATION
PLEWA, MJ
WAGNER, ED
YU, TW
ANDERSON, D
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 1995, 26 (02) : 171 - 177
[4] METABOLIC-ACTIVATION OF MUTAGENIC N-HYDROXYARYLAMINES BY O-ACETYLTRANSFERASE IN SALMONELLA-TYPHIMURIUM TA98
SAITO, K
SHINOHARA, A
KAMATAKI, T
KATO, R
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1985, 239 (01) : 286 - 295
[5] MUTAGENICITY OF SOME HETEROCYCLIC AMINES IN SALMONELLA-TYPHIMURIUM WITH METABOLIC-ACTIVATION BY HUMAN RED-BLOOD-CELL CYTOSOL
DUVERGERVANBOGAERT, M
CRUTZENFAYT, MC
STECCA, C
MUTATION RESEARCH, 1991, 261 (04): : 261 - 265
[6] MUTAGENICITY OF ANTIVIRAL SUBSTANCES OF NUCLEOBASE ANALOG TYPE IN SALMONELLA-TYPHIMURIUM EMPLOYING METABOLIC-ACTIVATION BY MOUSE-LIVER HOMOGENATE OR CELL-FREE PLANT-EXTRACTS
ELTARRAS, A
BRAUN, R
STENZ, E
SCHUSTER, G
ZENTRALBLATT FUR MIKROBIOLOGIE, 1989, 144 (03): : 191 - 196

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