EUGENOL CYTOTOXICITY EVALUATED WITH CONTINUOUS CELL-LINES

被引：27
|
作者：
BABICH, H
STERN, A
BORENFREUND, E
机构：
[1] NYU MED CTR,DEPT PHARMACOL,NEW YORK,NY 10016
[2] ROCKEFELLER UNIV,LAB ANIM RES CTR,NEW YORK,NY 10021
来源：
TOXICOLOGY IN VITRO | 1993年 / 7卷 / 02期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1016/0887-2333(93)90119-P
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
The cytotoxicity of eugenol to replicating cells, as mediated by the intracellular level of glutathione and by metabolic activation, was evaluated with the neutral red (NR) assay. The cytotoxicity of eugenol to human HFF fibroblasts and human HepG2 hepatoma cells was increased somewhat in the presence of a hepatic S-9 microsomal fraction from Aroclor-induced rats or hamsters. Exposure of human HepG2 hepatoma cells to eugenol depleted the level of intracellular glutathione. Cells treated with 1-chloro-2,4-dinitrobenzene (CDNB) or buthionine sulphoximine (BSO), agents that deplete intracellular glutathione, were hypersensitive to eugenol. A 1-hr pretreatment with CDNB enhanced the cytotoxicity of eugenol, as did a 24-hr pretreatment with BSO. Intracellular glutathione levels were, apparently, significant in mediating the toxicity of eugenol.
引用
收藏
页码：105 / 109
页数：5
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