PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE SPECIFIC PHOSPHOLIPASE-C IN PHARBITIS-NIL MEMBRANES

被引：2
|
作者：
BONNER, PLR
PRIOR, SL
HETHERINGTON, AM
LUMSDEN, PJ
机构：
[1] Department of Applied Biology, University of Central Lancashire, Preston
[2] Institute of Environmental and Biological Sciences, Lancaster University, Lancaster
来源：
BIOLOGIA PLANTARUM | 1992年 / 34卷 / 5-6期
关键词：
D O I：
10.1007/BF02923581
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Phosphatidylinositol 4,5-bisphosphate specific phospholipase C has been detected in a membrane preparation from Pharbitis nil cotyledons. The enzyme has a pH optimum of 6.8 and activated by calcium ions, deoxycholate, phosphatidylinositol and phosphatidylethanolamine. The enzyme is inhibited to varying degrees by Tween 20, Triton X100, zinc, copper, cobalt and manganese ions and phosphatidylserine. G-protein activators do not affect the activity of Pharbitis nil phospholipase C. Analysis of the products of the reaction by HPLC shows inositol 1,4,5-trisphosphate from phospholipase C and inositol bisphosphate from inositol-1 and -5 phosphatase activity.
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页码：367 / 372
页数：6
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