KINETICS OF HYDROLYSIS OF TYPE-I, TYPE-II, AND TYPE-III COLLAGENS BY THE CLASS-I AND CLASS-II CLOSTRIDIUM-HISTOLYTICUM COLLAGENASES

被引:43
|
作者
MALLYA, SK
MOOKHTIAR, KA
VANWART, HE
机构
[1] FLORIDA STATE UNIV, DEPT CHEM, TALLAHASSEE, FL 32306 USA
[2] FLORIDA STATE UNIV, INST MOLEC BIOPHYS, TALLAHASSEE, FL 32306 USA
来源
JOURNAL OF PROTEIN CHEMISTRY | 1992年 / 11卷 / 01期
关键词
CLOSTRIDIUM-HISTOLYTICUM COLLAGENASE; INTERSTITIAL COLLAGENS; COLLAGEN SPECIFICITY; KINETICS OF HYDROLYSIS; COLLAGEN STRUCTURE;
D O I
10.1007/BF01025096
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The kinetics of hydrolysis of rat tendon type I, bovine nasal septum type II, and human placental type III collagens by class I and class II Clostridium histolyticum collagenases (CHC) have been investigated. To facilitate this study, radioassays developed previously for the hydrolysis of these [H-3]acetylated collagens by tissue collagenases have been adapted for use with the CHC. While the CHC are known to make multiple scissions in these collagens, the assays are shown to monitor the initial proteolytic events. The individual kinetic parameters k(cat) and K(M) have been determined for the hydrolysis of all three collagens by both class I and class II CHC. The specific activities of these CHC toward fibrillar type I and III collagens have also been measured. In contrast to human tissue collagenases, neither class of CHC exhibits a marked specificity toward any collagen type either in solution or in fibrillar form. The values of the kinetic parameters k(cat) and K(M) for the CHC are similar in magnitude to those of the human enzymes acting on their preferred substrates. Thus, the widely held view that the CHC are more potent collagenases is not strictly correct. As with the tissue collagenases, the local collagen structure at the cleavage sites is believed to play an important role in determining the rates of the reactions studied.
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页码:99 / 107
页数:9
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