CLONING AND CHARACTERIZATION OF TRYPSIN-INHIBITOR GENE FROM VIGNA-UNGUICULATA

被引：3
|
作者：
BIJOLA, V [1 ]
PATHRE, U [1 ]
NATH, P [1 ]
SANE, PV [1 ]
机构：
[1] NATL BOT RES INST,LUCKNOW 226001,UTTAR PRADESH,INDIA
来源：
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY | 1994年 / 3卷 / 02期
关键词：
COWPEA TRYPSIN INHIBITOR; PURIFICATION; AMPLIFICATION; PROTEIN EXPRESSION; SEQUENCING;
D O I：
10.1007/BF03321961
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A trypsin inhibitor, member of Bowman-Birk family, was isolated and purified from cowpea (Vigna unguiculata). Polyclonal antibodies were raised against cowpea trypsin inhibitor (CpTI) protein in rabbits. The gene for CpTI was amplified by polymerase chain reaction and cloned in a bacterial expression vector pVCATFR18416. The expression of CpTI protein in BL21 (DE3) strain of Escherichia coli. was confirmed by western blot studies. The CpTI gene was also sequenced and found to exhibit 100% homology with already published sequence of CpTI gene.
引用
收藏
页码：113 / 119
页数：7
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