DETECTION OF INDUCIBLE PROSTAGLANDIN-H SYNTHASE-2 IN CELLS IN THE EXUDATE OF RAT CARRAGEENAN-INDUCED PLEURISY

被引：39

作者：

HARADA, Y ^{[1
]}

HATANAKA, K ^{[1
]}

SAITO, M ^{[1
]}

MAJIMA, M ^{[1
]}

OGINO, M ^{[1
]}

KAWAMURA, M ^{[1
]}

OHNO, T ^{[1
]}

YANG, QS ^{[1
]}

KATORI, M ^{[1
]}

YAMAMOTO, S ^{[1
]}

机构：

[1] UNIV TOKUSHIMA,SCH MED,DEPT BIOCHEM,TOKUSHIMA 770,JAPAN

来源：

BIOMEDICAL RESEARCH-TOKYO | 1994年 / 15卷 / 02期

关键词：

D O I：

10.2220/biomedres.15.127

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Pleurisy was induced in rats by intrapleural injection of 0.2 ml of 2% lambda-carrageenin. Prostaglandin H synthase (PGHS)-2 was detected using immunoblot analysis in cells of the pleural exudate 5 h after carrageenin injection, and waned at 19 h. PGHS-1 was also detected, but was maintained at similar levels in cells before and after carrageenin injection. The level of PGHS-2 was markedly suppressed by pretreatment of rats with anti-inflammatory dose of dexamethasone (3 mg/kg, i.p.) 2 h before carrageenin injection. Furthermore, only PGHS-1, but not PGHS-2, was detected in the microsomal fraction of the lung, stomach and kidney of pleurisy-affected and non-affected normal rats. These results suggest the involvement of PGHS-2 in the prostanoid formation in the inflammatory site.

引用

页码：127 / 130

页数：4

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