DEVELOPMENT OF A WHOLE-BLOOD ASSAY TO MEASURE T-CELL RESPONSES TO LEPROSY - A NEW TOOL FOR IMMUNO-EPIDEMIOLOGIC FIELD STUDIES OF LEPROSY IMMUNITY

被引：73
|
作者：
WEIR, RE
MORGAN, AR
BRITTON, WJ
BUTLIN, CR
DOCKRELL, HM
机构：
[1] LEPROSY MISSION,ANANDABON LEPROSY HOSP,MYCOBACTERIOL RES LABS,KATMANDU,NEPAL
[2] UNIV SYDNEY,DEPT MED,SYDNEY,NSW 2006,AUSTRALIA
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 176卷 / 01期
基金：
英国医学研究理事会;
关键词：
WHOLE BLOOD; T CELL; LEPROSY; IFN-GAMMA; EPIDEMIOLOGY;
D O I：
10.1016/0022-1759(94)90353-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A whole blood assay is described to measure T cell mediated immune responses to leprosy and provide an alternative to the conventional lymphocyte transformation test. Optimal conditions were defined for the whole blood assay, and interferon-gamma measurement was found to be a more sensitive way of measuring responses than tritiated thymidine incorporation. The assay was shown to be useful for investigating responses to a range of leprosy antigens. A whole blood assay has the advantages of being quick, simple and requiring only a small volume of blood, making it more appropriate as an immuno-epidemiological field test in leprosy endemic areas.
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页码：93 / 101
页数：9
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