Continuous semi-solid cultivation for the production of cellulase by Trichoderma reesei mutants using a polyurethane foam carrier and a liquid medium

被引：2
|
作者：
Targonski, Z
Pielecki, J
机构：
[1] Agricultural University, Department of Food Technology Akademicka, Lublin
来源：
ACTA BIOTECHNOLOGICA | 1995年 / 15卷 / 03期
关键词：
D O I：
10.1002/abio.370150307
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The production of cellulase was investigated in semi-solid state culture using the immobilized mycelium of Trichoderma reesei mutants on polyurethane foam impregnated with lactose medium. An extremely high value of about 2.6 FPU/ml was reached after the cultivation of T. reesei D-78085 on a 0.5% lactose medium in continuous culture at a pH medium of 4.0 when a bioreactor with vertical polyurethane foam plates was used. The enzyme yield on lactose was 520 FPU/g of lactose metabolized in comparison with 160 FPU/g using a stirred tank bioreactor.
引用
收藏
页码：289 / 296
页数：8
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