DETERMINATION OF NICOTINAMIDE-ADENINE DINUCLEOTIDE AND THIAZOLE-4-CARBOXAMIDE-ADENINE DINUCLEOTIDE IN HUMAN-LEUKOCYTES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：3
|
作者：
SAUNDERS, PP [1 ]
ALVAREZ, E [1 ]
KANTARJIAN, HM [1 ]
机构：
[1] UNIV TEXAS, MD ANDERSON CANC CTR, DEPT HEMATOL, HOUSTON, TX 77030 USA
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 577卷 / 01期
关键词：
D O I：
10.1016/0378-4347(92)80596-I
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A high-performance liquid chromatographic assay for cellular nicotinamide-adenine dinucleotide and thiazole-4-carboxamide-adenine dinucleotide is presented that is appropriate for analysis of these dinucleotides in extracts of Ficoll-purified human leukemic cells. The separation, which is effected by reversed-phase chromatography. is highly reproducible and the limit of quantitation is as low as 10-15 pmol. The stability of these compounds in neutralized perchloric acid extracts is addressed and the applicability of the procedure to clinical specimens is demonstrated.
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页码：37 / 41
页数：5
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