TRANSFORMATION OF BACILLUS-SUBTILIS BY ELECTROPORATION

被引：10
|
作者：
STEPHENSON, M [1 ]
JARRETT, P [1 ]
机构：
[1] INST HORT RES,LITTLEHAMPTON BN17 6LP,W SUSSEX,ENGLAND
来源：
BIOTECHNOLOGY TECHNIQUES | 1991年 / 5卷 / 01期
关键词：
D O I：
10.1007/BF00152746
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Optimal conditions for the transformation of Bacillus subtilis by electroporation were achieved. Frequencies of greater than 10(5) transformants/mu-g of plasmid DNA were obtained for a number of strains and plasmids. Increased transformation efficiency of mini-prep DNA from B. subtilis and Escherichia coli was obtained after microdialysis.
引用
收藏
页码：9 / 12
页数：4
相关论文
共 50 条
  • [1] FACTORS AFFECTING THE ELECTROPORATION OF BACILLUS-SUBTILIS
    MCDONALD, IR
    RILEY, PW
    SHARP, RJ
    MCCARTHY, AJ
    [J]. JOURNAL OF APPLIED BACTERIOLOGY, 1995, 79 (02): : 213 - 218
  • [2] GENETIC-TRANSFORMATION OF INTACT-CELLS OF BACILLUS-SUBTILIS BY ELECTROPORATION
    BRIGIDI, P
    DEROSSI, E
    BERTARINI, ML
    RICCARDI, G
    MATTEUZZI, D
    [J]. FEMS MICROBIOLOGY LETTERS, 1990, 67 (1-2) : 135 - 138
  • [3] PLASMID TRANSFORMATION IN BACILLUS-SUBTILIS
    CANOSI, U
    TRAUTNER, TA
    [J]. HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1979, 360 (08): : 1021 - 1021
  • [4] THE EFFECT OF CHLORPROMAZINE ON TRANSFORMATION IN BACILLUS-SUBTILIS
    MOOIBROEK, H
    VENEMA, G
    [J]. MOLECULAR & GENERAL GENETICS, 1982, 185 (01): : 165 - 168
  • [5] TRANSFORMATION OF BACILLUS-SUBTILIS BY STREPTOCOCCAL PLASMIDS
    BOITSOV, AS
    GOLUBKOV, VI
    [J]. DOKLADY AKADEMII NAUK SSSR, 1980, 254 (02): : 490 - &
  • [6] STIMULATION OF BACILLUS-SUBTILIS TRANSFORMATION BY SPERMIDINE
    CLARK, PO
    LEACH, FR
    [J]. MOLECULAR & GENERAL GENETICS, 1980, 178 (01): : 21 - 25
  • [7] Transformation of undomesticated strains of Bacillus subtilis by protoplast electroporation
    Romero, Diego
    Perez-Garcia, Alejandro
    Veening, Jan-Willem
    de Vicente, Antonio
    Kuipers, Oscar P.
    [J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2006, 66 (03) : 556 - 559
  • [8] PLASMID MARKER RESCUE TRANSFORMATION IN BACILLUS-SUBTILIS
    WEINRAUCH, Y
    DUBNAU, D
    [J]. JOURNAL OF BACTERIOLOGY, 1983, 154 (03) : 1077 - 1087
  • [9] TRANSFORMATION IN BACILLUS-SUBTILIS - A SIMPLE DIRECT METHOD
    AHARONOWITZ, Y
    RON, EZ
    [J]. MOLECULAR AND GENERAL GENETICS, 1970, 107 (01): : 94 - +
  • [10] SUR LA TRANSFORMATION DE BACILLUS-SUBTILIS
    SCHAEFFER, P
    IONESCO, H
    [J]. COMPTES RENDUS HEBDOMADAIRES DES SEANCES DE L ACADEMIE DES SCIENCES, 1959, 249 (03): : 481 - 482
12345