MICROTUBULE TRANSLOCATION CAUSED BY 3 SUBSPECIES OF INNER-ARM DYNEIN FROM CHLAMYDOMONAS FLAGELLA

被引：25
|
作者：
KAGAMI, O [1 ]
TAKADA, S [1 ]
KAMIYA, R [1 ]
机构：
[1] NAGOYA UNIV,SCH SCI,DEPT MOLEC BIOL,NAGOYA 46401,JAPAN
来源：
FEBS LETTERS | 1990年 / 264卷 / 02期
关键词：
Dynein; Microtubule; Motility; Translocation;
D O I：
10.1016/0014-5793(90)80243-C
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To help understand the function of inner-arm dynein in nagellar motility, dynein samples from an outer arm-missing mutant of Chlamydomonas (odal) were examined for the ability to translocate microtubules in vitro. High-salt extract ofaxonemes containing inner-arm dynein was separated by ion-exchange chromatography into 7 peak fractions with ATPase activities. Of these, three fractions containing different sets of dynein heavy chains translocated microtubules. The maximal velocities were all between 3 and 5 μm s, which were comparable to the microtubule sliding rate in disintegrating oda axonemes. © 1990.
引用
收藏
页码：179 / 182
页数：4
相关论文
共 50 条
  • [1] Processive movement of the inner-arm dynein subspecies c of Chlamydomonas flagella
    Kikumoto, M
    Nakamori, R
    Kojima, H
    Sakakibara, H
    Oiwa, K
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2004, 15 : 154A - 154A
  • [2] Inner-arm dynein subspecies C of Chlamydomonas flagella is a single-headed processive motor.
    Sakakibara, H
    Sakai, Y
    Kojima, H
    Yamaga, T
    Oiwa, K
    [J]. MOLECULAR BIOLOGY OF THE CELL, 1998, 9 : 155A - 155A
  • [3] TRANSLOCATION AND ROTATION OF MICROTUBULES CAUSED BY MULTIPLE SPECIES OF CHLAMYDOMONAS INNER-ARM DYNEIN
    KAGAMI, O
    KAMIYA, R
    [J]. JOURNAL OF CELL SCIENCE, 1992, 103 : 653 - 664
  • [4] One-dimensional diffusion along a microtubule of the inner-arm dynein subspecies c of Chlamydomonas
    Kikumoto, M
    Nakamori, R
    Kojima, H
    Sakakibara, H
    Oiwa, K
    [J]. BIOPHYSICAL JOURNAL, 2005, 88 (01) : 652A - 653A
  • [5] Ratchetlike Properties of In Vitro Microtubule Translocation by a Chlamydomonas Inner-Arm Dynein Species c in the Presence of Flow
    Kikushima, Kenji
    Kamiya, Ritsu
    [J]. BIOPHYSICAL JOURNAL, 2009, 97 (06) : 1657 - 1662
  • [6] Inner-arm dynein c of Chlamydomonas flagella is a single-headed processive motor
    Hitoshi Sakakibara
    Hiroaki Kojima
    Yukako Sakai
    Eisaku Katayama
    Kazuhiro Oiwa
    [J]. Nature, 1999, 400 : 586 - 590
  • [7] Inner-arm dynein c of Chlamydomonas flagella is a single-headed processive motor
    Sakakibara, H
    Kojima, H
    Sakai, Y
    Katayama, E
    Oiwa, K
    [J]. NATURE, 1999, 400 (6744) : 586 - 590
  • [8] Mechanical properties of Chlamydomonas inner-arm dynein subspecies measured by optical trapping nanometry
    Kojima, H
    Sakakibara, H
    Sakai, Y
    Yamaga, T
    Oiwa, K
    [J]. BIOPHYSICAL JOURNAL, 1999, 76 (01) : A46 - A46
  • [9] Diversity and similarity of mechnochemical properties of Chlamydomonas inner-arm dynein
    Sakakibara, H
    Sakai, Y
    Kotani, N
    Kikumoto, M
    Oiwa, K
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2000, 11 : 194A - 194A
  • [10] Mechanical properties of a single-headed processive motor: Inner-arm dynein subspecies c of chlamydomonas flagella studied at the single molecule level.
    Kojima, H
    Sakakibara, H
    Sakai, Y
    Kikumoto, M
    Oiwa, K
    [J]. BIOPHYSICAL JOURNAL, 2000, 78 (01) : 125A - 125A
12345