Efficacy of Crude Alcoholic Extracts from Culture Broth of Desert Truffles against Some Clinical Microbial Isolates

This work amid to study the microscopic and macroscopic features of desert truffles cultivated on potato dextrose agar and broth. Also evaluation of crude alcoholic extracts of primary and secondary metabolisms against some clinical microorganisms isolates. The white truffles (Al-kama'a or Al-faga'a) locally known as "Zubaidi" collected from Riyadh markets. Isolation of ascospores and purity of ascospores test were performed after that the ascospores of truffles was cultivated on PDA and PDB. The microscopic and macroscopic were studied. The fermentation was carried out by four several conditions. After extraction by ethyl acetate and ethanol, the extracts were evaluated as anti-microbial agents. The results indicated that the desert truffles ascospores grew on PDA and PDB and the mycelia and macrospores were produced. The colony was white cottony with bloc in center without any pigment in front or reverse plate. A hyaline septate hyphae, conidiophore on both sides of hyphae, slightly ellipsoid macro-conidia and like of chlamydospores at end of some conidiophore were observed. The extracts [Ethyl acetate (CEA) and ethanol (CE)] from cultures that were grown in 12 hours light and 12 hours dark without shaker had higher effective against S. mutans, Shigella spp., Cr. neoformans and C. albicans. The results show that all extracts had not any activity against A. niger. The work suggested that primary and secondary metabolisms extracts of desert truffles grown on laboratory could be renewable resource of a many of biological compounds.